Application

Western blot: 1:2,000-1:5,000 using ECL. Reacts with the ~45 kDa IDO protein. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 with 5% skim milk. Suggested incubation time is 1 hour at room temperature.

ELISA, direct and indirect: 1:2,000-1:10,000.

Optimal working dilutions must be determined by the end user.

Anti-IDO Antibody detects level of IDO & has been published & validated for use in ELISA & WB.

Immunogen

Recombinant mouse IDO.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-1056

Physical form

Format: Purified

Liquid on PBS, pH 7.4.

Specificity

IDO [Indoleamine 2,3-dioxygenase], mouse.

Target description

45 kDa